Equipment

BD LSR II Flow Cytometer System

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bd lsr ii flow cytometer system

Lasers
Blue laser, 488nm
Red Laser, 633nm
Detectors
Forward Scatter detector is a photodiode
Five wave lengths and side scatter are detected from the 488-nm laser
530/30 nm (FITC, Alexa Fluor 488, GFP, CFSE, Calcein, YFP, R123, Mito Green, Syto 14-18, YoPro3, Fluo4)
575/26 nm (PE)
610/20 nm (PE-TexasRed, Propidium Iodide, PE-Alexa Flour 610)
695-40 nm (PE-Cy5.5, PerCP-Cy5.5, PE-Alexa Flour 700)
780/60 nm (PE-Cy7)
Three wave lengths are detected from the 633-nm laser
660/20 nm (APC)
710/50 nm (Alexa Fluor 700)
780/90 nm (APC-Cy7, APC-Alexa Fluor 750, APC-H7)

From BD Biosciences

This page last updated 23rd May 2013